Pyrogen-isina consumables inodyiwa isina exogenous endotoxin, inosanganisira pyrogen-isina pipette matipi (tip bhokisi), pyrogen-isina test tubes kana inonzi endotoxin isina magirazi machubhu, pyrogen-isina girazi ampoules, endotoxin-isina 96-tsime microplates, uye endotoxin- mvura yakasununguka (depyrogenated mvura inoshandiswa mubhakitiriya endotoxin test), endotoxin isina buffer uye nezvimwewo Pakati pavo, mvura yebhakitiriya endotoxintest negel clot nzira uye quantitative endotoxin test assay mune zvose zvine chokuita nePharmacopoeia editions (USP, EP, BP, JP uye China Pharmaceutica).Zvinoreva mvura isina utachiona yejekiseni ine endotoxin yemukati isingasviki 0.015EU/ml.Ikozvino iyo yazvino vhezheni yePharmacopoeia, iyo BET mvura isingasviki 0.005EU/ml.Kunyangwe iyo yepamusoro mwero isingasviki 0.001EU/ml inokwanisa kugadzirwa uye kupihwa neBioendo.
Ane hukama akakosha mazwi e endotoxins, uye endotoxin yemahara consumables, pyrogen uye kupisa masosi ari maviri akasiyana pfungwa:Pyrogen: Uyewo inonzi pyrogen kana exothermic factor.zvinhu zvinogona kukonzera kuwedzera kwekushisa kwemuviri.Heat Source: Chinhu chinoburitsa kupisa.Zvakadai semachisi ekupisa, marasha nezvimwe.Izvo zvinonzi "zvisiri-pyrogenic consumables" uye "pyrogenic response" yevamwe vanogadzira uye vatengesi chaizvoizvo mazita asina ruzivo uye anotsausa.Izvo zvakakodzera zvinofanirwa kunge zviri "Pyrogen Yemahara" uye "Pyrogen Response".
Nei Pyrogen yemahara inodyiwa ichidikanwa muye endotoxin bvunzo assay, ese ari maviri gel clot endotoxin test assay uye kuwanda endotoxin test assay?
Ehe, pyrogen-isina consumables yakakosha pakuita endotoxin bvunzo assay nemazvo uye nekuvimbika.Kuvapo kwepyrogens, izvo zvinopisa kupisa-inducing zvinhu zvinowanzobva kune mabhakitiriya endotoxins, zvinogona kupindira nemigumisiro yekuedzwa uye kutungamirira kuverenga kwenhema.Iyo endotoxin bvunzo, inowanzo zivikanwa seLimulus amebocyte lysate (LAL) bvunzo kana inonzi Lyophilized amebocyte lysate (LAL) bvunzo, inoshandiswa kuona uye kuyera kuvepo kwebhakitiriya endotoxins mumishonga, michina yekurapa, uye zvimwe zvigadzirwa.Iyo LAL bvunzo inovimba nekuita pakati peLAL reagent uye endotoxins kuburitsa inogwamba kana chromogenic mhinduro.Kuti uve nechokwadi chemhedzisiro, zvakakosha kushandisa zvinodyiwa zvisina pyrogens.Pyrogens inogona kusvibisa zvinhu zvakasiyana-siyana zverabhoritari, kusanganisiraglassware, pipette matipi, machubhu, uye midziyo yekuenzanisira.Kana pyrogen-yakasvibiswa zvinodyiwa zvikasangana neLAL reagent kana bvunzo sampuli, zvinogona kukonzeresa manyepo-zvakanaka maitiro, zvichitungamira kune mhedziso dzisiridzo pamusoro pekuvapo kana kuunganidzwa kweendotoxins.Pyrogen-isina consumables inogadzirwa uye inoedzwa zvakananga kuderedza kana kubvisa kuvapo kwemapyrogens.Ivo vanopinda zvakaomesesa zvemhando yekudzora maitiro kuti vave nechokwadi chekuti vanosangana nezvinodiwa zvekuyedzwa kwe endotoxin.Kushandisa izvi zvekushandisa zvine hunyanzvi kunobatsira kuchengetedza kutendeseka uye kurongeka kweiyo endotoxin test assay, kuve nemhedzisiro yakavimbika uye nekusimudzira kuchengetedzwa kwevarwere mumishonga uye biomedical application.
Nguva yekutumira: Nov-07-2022
