Endotoxin-Isina Mvura: Kuita Basa Rinokosha muEndotoxin Test Assays
Nhanganyaya:
Endotoxin kuyedzwa chinhu chakakosha mumaindasitiri akasiyana, anosanganisira emishonga, mudziyo wekurapa, uye biotechnology.Iyo chaiyo uye yakavimbika yekuona endotoxins kwakakosha kuve nechokwadi chekuchengetedzwa kwechigadzirwa uye kutevedzera mitemo.Chimwe chakakosha chinodiwa pakuita endotoxin bvunzo kushandisa endotoxin-isina mvura.Muchinyorwa chino, tichaongorora kukosha kwemvura isina endotoxin, basa rayo mukuita Lyophilized Amebocyte Lysate (LAL) endotoxin bvunzo, uye kukosha kwekushandisa endotoxin-isina mvura muBacterial Endotoxin Test (BET).
Kunzwisisa Endotoxins:
Endotoxins i lipopolysaccharides (LPS) inowanikwa pa membrane yekunze yeGram-negative bacteria.Ivo varevereri vane simba vekuzvimba uye vanogona kukonzera mhedzisiro yakaipa kana iripo muzvigadzirwa zvemishonga kana michina yekurapa.Nekuda kwekugona kwavo kukonzera pyrogenic reactions, kunyatsoona uye quantification ye endotoxins yakakosha.
LAL Endotoxin Testing:
Nzira inonyanya kuzivikanwa yekuongororwa kwe endotoxin ndeye LAL assay, iyo inoshandisa ropa rehorshoe crab.Limulus polyphemus uye Tachypleus tridentatus.Lyophilized Amebocyte Lysate (LAL) reagent inotorwa kubva mumasero eropa emakakanje aya, ane puroteni inovhara iyo inogadziriswa pamberi peendotoxins.
Basa reEndotoxin-Isina MvuramuLAL Testing:
Mvura chinhu chekutanga mukugadzirira reagent uye nhanho dzekudzikisa yeLAL yekuyedza.Nekudaro, kunyangwe kuteedzera huwandu hweendotoxins huripo mumvura yepombi yenguva dzose inogona kukanganisa kurongeka uye kunzwisiswa kweiyo assay.Kukunda dambudziko iri, endotoxin-isina mvura inofanirwa kushandiswa panguva yese yekuyedza.
Endotoxin-isina mvura inoita basa rakakosha mukuona kuti mareagents anoshandiswa muLAL assay haana kusvibiswa ne endotoxins.Uyezve, inodzivirira nhema dzakanaka kana nhema dzisina kunaka mhedzisiro, nekudaro ichipa yakavimbika uye chaiyo endotoxin quantification.
Kusarudza Mvura Yakakodzera Yekuyedzwa kweLAL:
Kuti uwane endotoxin-isina mvura, akati wandei nzira dzekuchenesa dzinogona kushandiswa.Deionization, distillation, uye reverse osmosis inowanzoshandiswa nzira kuderedza kuvapo kwe endotoxins mumvura.Aya maitiro anobvisa tsvina dzakasiyana, kusanganisira endotoxins inotorwa kubva kubhakitiriya.
Pamusoro pezvo, zvakakosha kuve nechokwadi chekuti midziyo inoshandiswa kuchengetedza, kuunganidza, uye kugovera endotoxin-isina mvura yakasimbiswa nemazvo uye isina kusvibiswa kwe endotoxin.Izvi zvinosanganisira kushandisa endotoxin-isina machubhu, mabhodhoro, uye mafirita panguva yekuita.
Kukosha kweBET Mvura:
MuBacterial Endotoxin Test (BET), endotoxin-isina mvura, inozivikanwawo seBET mvura, inoshandiswa sekutonga kusina kunaka kusimbisa kunzwisiswa uye kujekesa kweLAL assay.Mvura yeBET inofanirwa kunge iine mwero usingaonekwe we endotoxins, kuve nechokwadi chekuti chero chinoyerwa endotoxin chiitiko chinobva chete kubva kumuenzaniso wakaedzwa.
Kushandiswa kwemvura yeBET muyedzo ye endotoxin kunoshanda sekutonga kwakakosha kuratidza kushanda kweLAL reagents, test system, uye michina.Iyi nhanho yekusimbisa yakakosha kunyatso ongorora kuvepo uye kusungirirwa kwe endotoxins mumuenzaniso wakaedzwa.
Mhedziso:
Endotoxin-isina mvura inoita basa rakakosha mukuonekwa kwakaringana uye kwakavimbika kwe endotoxins mumaindasitiri akasiyana.Mukuyedzwa kweLAL endotoxin, inova nechokwadi chekuti mareagents anoshandiswa haana kusvibiswa, achipa chaiyo quantification.MuBET, endotoxin-isina mvura inoshanda sekutonga, ichisimbisa kunzwisiswa kweLAL assay.Nekuomerera kune dzakaomarara nzira dzekuchenesa uye kushandisa midziyo yakasimbiswa, mukana wemhedzisiro uye zvikanganiso zvinogona kudzikiswa zvakanyanya.
Sezvo kukosha kwekuyedzwa kwe endotoxin kuri kuramba kuchikura, basa remvura isina endotoxin rinowedzera kukosha.Kushandisa nzira dzinovimbika dzekuchenesa mvura uye nekubatanidza maitiro akanakisa muyedzo maitiro kunovimbisa kuchengetedzwa uye kutevedza kwezvigadzirwa zvemishonga, michina yekurapa, uye zvimwe zvinhu zvinonzwa endotoxin.
Nguva yekutumira: Nov-29-2023
