Chii chinonzi Endotoxins Test?
Endotoxins ma hydrophobic molecules ari chikamu che lipopolysaccharide complex iyo inoumba yakawanda yekunze membrane yeGram-negative bacteria.Vanosunungurwa kana mabhakitiriya afa uye membrane yavo yekunze inoparara.Endotoxins inoonekwa seyakanyanya kupa kune mhinduro yepyrogenic.Uye zvigadzirwa zvevabereki zvakasvibiswa nemapyrogens zvinogona kutungamirira mukukura kwefivha, induction yekuzvimba mhinduro, kuvhunduka, kutadza kwenhengo uye kufa muvanhu.
Endotoxins bvunzo ndiyo bvunzo yekuona kana kuverengera endotoxins kubva kuGram-negative bacteria.
Tsuro dzinoshandiswa kuona uye kuyera endotoxins muzvigadzirwa zvemishonga pakutanga.Sekureva kweUSP, iyo RPT inosanganisira kutarisa kuwedzera kwekushisa kana fivha mushure mejekiseni remutsinga wemishonga mutsuro.Uye 21 CFR 610.13(b) inoda bvunzo yepyrogen yetsuro yezvigadzirwa zvebiology.
Mumakore ekuma1960, Fredrick Bang naJack Levin vakawana kuti amebocytes egakanje rebhiza anogwamba pamberi peendotoxins.TheLimulus Amebocyte Lysate(kana Tachypleus Amebocyte Lysate) yakagadziridzwa zvinoenderana nekutsiva yakawanda RPT.PaUSP, bvunzo yeLAL inodaidzwa seiyo bhakitiriya endotoxin bvunzo (BET).Uye BET inogona kuitwa nekushandisa nzira nhatu: 1) gel-clot technique;2) nzira ye turbidimetric;3) nzira yechromogenic.Zvinodiwa zveLAL bvunzo ine yakakwana pH, ionic simba, tembiricha, uye nguva yekufukidzira.
Kuenzaniswa neRPT, BET inokurumidza uye inoshanda.Nekudaro, BET haina kukwanisa kutsiva RPT zvachose.Nekuti LAL assay inogona kukanganiswa nezvinhu uye haikwanise kuona isiri-endotoxin pyrogens.
Nguva yekutumira: Dec-29-2018
