Endotoxins madiki mabhakitiriya-anotorwa hydrophobic lipopolysaccharides (LPS) mamorekuru anowanikwa kunze kwesero membrane ye gram-negative bacteria.Endotoxins ine core polysaccharide chain, O-specific polysaccharide side cheni (O-antigen) uye lipid compenent, Lipid A, iyo inokonzeresa mhedzisiro yehupfu.Utachiona hunodurura endotoxin muhuwandu huzhinji pakufa kwesero uye pavanenge vachikura nekuparadzanisa.Imwe Escherichia coli ine mamorekuru maviri eLPS pasero.
Endotoxin inogona kusvibisa ma labwares zviri nyore, uye kuvapo kwayo kunogona kupa zvakanyanya mu vitro uye mu vivo kuyedza.Uye kune zvigadzirwa zvevabereki, zvigadzirwa zvevabereki zvakasvibiswa ne endotoxins zvinosanganisira LPS zvinogona kutungamira mukukura kwefivha, induction yekuzvimba mhinduro, kuvhunduka, kutadza kwenhengo uye kufa mumunhu.Kune zvigadzirwa zve dialysis, LPS inogona kutamiswa kuburikidza ne membrane ine hombe pore saizi nekumashure-kusefa kubva ku dialysis fluid kuenda kuropa, matambudziko ekuzvimba anogona kukonzerwa saizvozvo.
Endotoxin inowanikwa neLyophilized Amebocyte Lysate (TAL).Bioendo yakazvipira kutsvagisa, kugadzira uye kugadzira TAL reagent kweanopfuura makumi mana emakore.Zvigadzirwa zvedu zvinofukidza matekiniki ese anoshandiswa kuona endotoxin, ari gel-clot tekinoroji, turbidimetric tekinoroji, uye chromogenic maitiro.
Nguva yekutumira: Jan-29-2019
