Recombinant Factor C (rFC) bvunzoinzira yakashandiswa zvikuru yekuona bhakitiriya endotoxins, inozivikanwawo se lipopolysaccharides (LPS), Endotoxins inhengo yekunze membrane yeGram-negative bacteria inogona kukonzera mhinduro yakasimba yekuputika mumhuka, kusanganisira vanhu.Iyo rFC assay yakavakirwa pakushandiswa kweiyo genetically engineered fomu yeFactor C, enzyme inongowanikwa muropa remabhiza uye inobatanidzwa munzira yekugwamba.Mune rFC assay, iyo recombinant Factor C inoshandiswa kuona kuvepo kwe endotoxins nekuyera Nekuyera zviri mukati meiyo cleaved substrates pamberi peendotoxin.Kuenzaniswa nemaitiro echinyakare endotoxin yekuona, senge Limulus Amebocyte Lysate (LAL) assay iyo inoshandisa horseshoe gakanje ropa, iyo rFC assay inoonekwa seyakanyanya kurongeka uye kudzokororwa, sezvo isingavimbe nekushandiswa kwemhuka-yakagadzirwa reagents.Iyo rFC assay zvakare yakawedzera hushamwari kwezvakatipoteredza uye yakagadzikana, sezvo ichideredza kudiwa kwekuunganidza uye kushandiswa kwemabhiza emabhiza mukuonekwa kwe endotoxin.
Iyo rFC assay inotenderwa nezviremera zvinodzora, zvakaita seUnited States Pharmacopeia (USP), European Pharmacopoeia (EP) uye Chinese Pharmacopoeia (CP) kuti ishandiswe mukuyedzwa kwemhando yekudzora kwemishonga nemidziyo yekurapa.
Zvakanakira recombinant factor c assay
Iyo Recombinant Factor C (rFC) assay inopa akawanda mabhenefiti pamusoro pechinyakare nzira dzekuona endotoxins, senge Limulus Amebocyte Lysate (LAL) assay.Mamwe mabhenefiti eiyo rFC assay anosanganisira:
1. Kumira: The rFC assay inyanzvi yeDNA inoshandiswa inoshandisa imwe chete, inotsanangurwa puroteni seyero yekuona.Izvi zvinoita kuti muyedzo uwedzere kurongeka uye usatadze kuchinjika kana uchienzaniswa neLAL assay, iyo inotsamira pakushandiswa kwemusanganiswa wakaoma wemapuroteni anotorwa kubva muropa rehove.
2. Reproducibility: The rFC assay ine mazinga akakwirira ekubereka, sezvo inoshandisa imwe chete, inotsanangurwa puroteni seyero yekuona.Izvi zvinobvumira mhedzisiro inowirirana, kunyangwe pamabhechi akasiyana uye akawanda mareagents.
3. Kuderedza kushandiswa kwemhuka: The rFC assay inzira inochengetedza zvakatipoteredza uye yakagadzikana yekuona endotoxins, sezvo isingadi kushandiswa kwemhuka mhenyu kana zvibayiro, zvakadai sehoveshoe crabs.
4. Inodhura-inoshanda: The rFC assay kazhinji inodhura-inoshanda kupfuura yeLAL assay, nekuda kwekuderedzwa kudiwa kwemhuka mhenyu uye zvakanyanya kuenderana maitiro echiyedzo.
5. Kugadzikana: The rFC assay yakasimba uye inogona kushandiswa mune zvakasiyana-siyana zvekushandisa, kusanganisira kuongororwa kwehutano hwehutano hwemishonga, zvigadzirwa zvemishonga, uye zvimwe zvigadzirwa zvingave zvine endotoxins.
6. Mvumo yemitemo: The rFC assay yakatenderwa nevakuru vane masimba vakaita seUnited States Pharmacopeia (USP) , European Pharmacopoeia (EP) uye Chinese Pharmacopoeia (CP) kuti ishandiswe mukuongororwa kwehutano hwehutano hwemishonga nemishonga yezvokurapa.Izvi zvinopa chivimbo chepamusoro mukuvimbika uye kurongeka kwekuongorora.
Kuti isangane nekusiyana kwezvinodiwa, Bioendo inogadzirawo uye inopa nzira yechinyakare yegel clot endotoxin test assay kit, yekukurumidza gel clot assay kit, quantitative endotoxin test assay kit inosanganisira "kinetic turbidimetric endotoxin test assay kituyekinetic chromogenic endotoxin test assay kit” .
Nguva yekutumira: Feb-19-2023
