LAL Reagent kana TAL Reagent ye endotoxin test assay

Limulus amebocyte lysate (LAL) kana Tachypleus tridentatus lysate (TAL) imvura inotorwa kubva mumasero eropa kubva kuhoveshoe crab.

Uye endotoxins ma hydrophobic molecules ari chikamu che lipopolysaccharide complex iyo inoumba yakawanda yekunze membrane yeGram-negative mabhakitiriya.Zvigadzirwa zvevabereki zvakasvibiswa nemapyrogens zvinogona kutungamira kune zvakakomba senge fivha, kuvhunduka, kutadza kwenhengo, kana kufa.

LAL/TAL reagent inogona kuita nebhakitiriya endotoxin uye lipopolysaccharide (LPS).Endotoxin yeLAL inosunga uye kugona kugwamba ndiyo inoita kuti ive yakakosha kuindasitiri yedu yemishonga.Uye ichi ndicho chikonzero LAL/TAL reagent inogona kushandiswa kuona kana kuyera bhakitiriya endotoxin.

Kusati kwaonekwa kuti LAL/TAL inogona kushandiswa kuita bvunzo yehutachiona endotoxins, tsuro dzinoshandiswa kuona uye kuverengera endotoxins muzvigadzirwa zvemishonga.Kana ichienzaniswa neRPT, BET neLAL/TAL reagent inokurumidza uye inoshanda, uye ndiyo nzira yakakurumbira yekuita inosimba yekutarisa endotoxin kusungwa muindasitiri yemishonga, zvichingodaro.

Iyo gel clot endotoxin test assay, inozivikanwawo seLimulus Amebocyte Lysate (LAL) bvunzo, kana inonzi Lyophilized Amebocyte Lysate (LAL) inzira inoshandiswa zvakanyanya yekuona uye kuverengera endotoxins muzvigadzirwa zvakasiyana, kunyanya mumaindasitiri emishonga nemidziyo yekurapa.Inoonekwa semhinduro inodiwa mumunda wekuonekwa kwe endotoxin nekuda kwekuita kwayo uye kugamuchirwa kwemutemo.

Muedzo weLAL unobva pamusimboti wekuti masero eropa egakanje remabhiza (Limulus polyphemus kana Tachypleus tridentatus) ane chinhu chinogwamba chinopindirana nemabhakitiriya endotoxins, zvichikonzera kuumbwa kwegel-like clot.Kuita uku kunonzwa zvakanyanya uye kwakananga kune endotoxins, izvo zvine chepfu zvikamu zvekunze membrane ye gram-negative bacteria.

Pane zvikonzero zvakati kuti nei gel clot endotoxin test assay ichionekwa semhinduro inodiwa mukuonekwa kwe endotoxin:

1. Regulatory Acceptance: Muedzo weLAL unozivikanwa uye unogamuchirwa nezviremera zvinodzora zvakadai seUnited States Pharmacopeia (USP) uye European Pharmacopoeia (EP) senzira yakajairika yekuongorora endotoxin.Kutevedzwa nemirairo iyi kunosungirwa kuve nechokwadi chekuchengetedza uye kunaka kwezvigadzirwa zvemishonga.

2. Sensitivity uye Specificity: Muedzo weLAL une kunzwisisika kwakanyanya, zvichiita kuti pave nekuonekwa kwemazinga akaderera endotoxins.Inokwanisa kuona endotoxin concentrations yakaderera se 0.01 endotoxin units per milliliter (EU/mL).Iyo chaiyo yebvunzo inovimbisa kuti inonyanya kuona endotoxins uye inoderedza nhema-yakanaka mhedzisiro.

3. Mutengo-Kubudirira: Iyo gel clot endotoxin test assay inowanzoonekwa semhinduro yehupfumi kana ichienzaniswa nedzimwe nzira dzakadai sechromogenic kana turbidimetric assays.Zvinoda mashoma mareagents uye zvishandiso, kuderedza mutengo wekuyedza.Pamusoro pezvo, kuwanikwa kweakamisikidzwa LAL reagents pamusika kunoita kuti zvive nyore kuti marabhoritari aite bvunzo.

4. Indasitiri Standard: Muedzo weLAL wakagamuchirwa zvakanyanya mumaindasitiri emishonga nemidziyo yekurapa senzira yakajairwa yekuona endotoxin.Icho chikamu chakakosha chemaitiro ekutonga kwemhando panguva yekugadzirwa kwezvigadzirwa zvemishonga nemidziyo yekurapa, kuve nechokwadi chekuteedzera zvinodiwa zvekutonga.

Nekudaro, zvakakosha kucherechedza kuti iyo gel clot endotoxin test assay inogona kunge iine painogumira, senge kukanganiswa kubva kune zvimwe zvinhu uye mukana wemanyepo-akanaka kana emanyepo-negative mhedzisiro.Mune zvimwe zviitiko, dzimwe nzira senge chromogenic kana turbidimetric assays dzinogona kushandiswa kuzadzisa kana kusimbisa zvawanikwa kubva kuLAL bvunzo.