Endotoxin Test Assay neLyophilized Amebocyte Lysate (LAL Reagent)

Endotoxin Test Assay neLyophilized Amebocyte Lysate (LAL Reagent)

LAL Reagents: Lyophilized amebocyte lysate (LAL) is aqueous extract of blood cells (amebocytes) kubva kuAtlantic horseshoe crab.
TAL Reagents: TAL reagent imvura inobuda yemasero eropa kubva kuTachypleus tridentatus.
Parizvino, kugadzirwa kukuru kweLAL/TAL reagents kuri muUnited States neChina.

Gel clot endotoxin test assay, iyi nzira ichiri ndiyo inonyanya kushandiswa pasi rese, kuitira kuti isangane nekuchengetedzwa kwemishonga yejekiseni reMunhu.
ZVINO Bioendo inogadzira uye inopa gel clot LAL reagent kusanganisira kune imwechete bvunzo girazi ampules uye akawanda bvunzo mabhodhoro.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 uye G52
Iyi ndiyo mhinduro yehupfumi yemhando yepamusoro endotoxins yekuona muyedzo yemushonga.Kunyanya kune iyo injectable zvinodhaka kana parenteral mishonga yekuedza endotoxins muWFI, API kana akapedza zvinodhaka zvigadzirwa.Endotoxin test assay ndeyekudiwa kwakanyanya kwemaitiro ekushanda, inoda hunyanzvi mushandisi kubata neiyo gel clot assay kuti ive nechokwadi chemhinduro dzakakwana.

Mhinduro yakakwana yeG52Endotoxin test assay:
LAL Reagent
Kudzora Standard Endotoxin
BET Mvura
Endotoxin-isina pipette mazano
Endotoxin-isina girazi machubhu, anosanganisira dilution oparesheni uye reaction machubhu.
Incubation mudziyo, kurudzira kugeza mvura kana yakaoma kupisa incubator.Yese incubation midziyo inoda tembiricha chaiyo.
Zvese zvinobatika kubata iyo LAL assay inosangana neyakajairwa endotoxin-isina level ” <0.005EU/ml ” .
Nzvimbo yekuyedza ichaenderana nekuona endotoxin.

Chenjerera ku:
Zvekushandisa zvepurasitiki senge pipette matipi kana multiwell mahwendefa anoshandiswa zvakanyanya kuyedza endotoxin.Iyo Harmonized Pharmacopoeias (USP/CP) inoda kuti chero zvinhu zvepurasitiki zvinodyiwa uye machubhu egirazi anofanirwa kunge asina endotoxin inooneka uye yakakosha zvakaenzana asi kazhinji isingatariswe, inofanirwa chiyero chemahara chezvinokanganisa.

Maitiro ekushandisa endotoxin bvunzo assay kuona endotoxins mumuenzaniso?
Chekutanga pane zvese, bvunzo yekusimbisa yakanyorwa lysate senitivity inofanira kuitwa.Kusimbisa kunzwisiswa kwakafanana kune ma label mamaki.
Kuti uongorore sampuli, ita pre-interference endotoxin test assays.
Kushandisa yakakwana endotoxin assay ye "kupindira bvunzo".
Kushandisa muyedzo wemuganho wekuwana sei iyo endotoxins nhanho mumuenzaniso.

Paunenge uchishanda bvunzo yekusimbisa yakanyorwa lysate senitivity, mhedzisiro yacho haina kujairika, 2 Lamada havenot gel formation?
Tarisa uone kana iyo nzira yekugadzirira yeiyo bhakitiriya yekudzora standard endotoxin ndeyechokwadi.
Kusanganiswa kweVortex kunodiwa kune yega yega diluent (ona bhuku rekuraira kana kunzi kupinza kweiyo control standard endotoxin mune ruzivo).
Kuti utarise kana iyo lysate reagent inoenderana neyakajairika endotoxin.

Inokurudzirwa kushandisa bhati remvura kana yakaoma inopisa incubator yekugara kwekushisa kweiyo lysate reagent (somuenzaniso, incubator kana bhokisi rakaoma harina kushanda).
Munguva yekunyungudika, nharaunda yemvura mumvura yekugezesa iyo inomira uye yakagadzikana, kudengenyeka kwese kunodziviswa.
Chengetedza zvakanyanya kugezera remvura kuti udzime pombi yekuyerera kwemvura.

Tichifunga mhedzisiro, zvinyoro tora chubhu yekuyedza kana girazi ampoule kubva kune thermostat, zvishoma nezvishoma invert 180 degrees,
iyo gel kuumbwa muchubhu haizoremara, uye haizotsvedza, mhedzisiro yakanyorwa nechiratidzo "+";
hapana kuumbwa kwegel kunoumbwa kana gel clot haigoni kuchengetwa yakasimba kunyange zvazvo yakaumbwa.
Kutsvedza kwemadziro hakuna kunaka, mhedzisiro yacho yakanyorwa nechiratidzo "-".
Rapid gel clot endotoxin test Kit ndeyeiyo gel clot endotoxin bvunzo nzira.
Mukukurumidza gel clot endotoxin kuyedza, iyo sampuli yakanaka yekudzora haiburitse gel?
Chekutanga, shandisa bhakitiriya endotoxin yekuyedza mvura kuti uone ma lysate reagents, ive nechokwadi chekuti vashandisi uye nharaunda vasangana nemirairo;

Kana iyo kiti ichikodzera, saka kutonga kwakanaka kwemuenzaniso hakuiti gel kuumbwa nekuda kweiyo inhibitory effect yemuenzaniso, uye sampuli inofanira kugadziriswa uye kurapwa.
Iyo yakajairika sampuli yekugadzirisa nzira ndeye dilution.