KCET- Kinetic Chromogenic Endotoxin Test Assay (Chromogenic endotoxin test assay inzira yakakosha yemasampuli nekumwe kukanganisa.)
Iyo kinetic chromogenic endotoxin bvunzo (KCT kana KCET) assay inzira inoshandiswa kuona kuvepo kwe endotoxins mumuenzaniso.
Endotoxins zvinhu zvine muchetura zvinowanikwa mumadziro esero emamwe marudzi emabhakitiriya, anosanganisira giremu-negative mabhakitiriya akadai seEscherichia coli uye Salmonella.MuKCET assay, chromogenic substrate inowedzerwa kumuenzaniso, iyo inopindirana nechero endotoxins iripo kuburitsa shanduko yeruvara.
Mwero wekukura kwemavara unotariswa nekufamba kwenguva uchishandisa spectrophotometer, uye huwandu hweendotoxin mumuenzaniso hunoverengerwa zvichienderana nechiyero ichi.
Iyo KCT assay inzira yakakurumbira yekuona endotoxins mumishonga, michina yekurapa, uye zvimwe zvigadzirwa zvinosangana nemuviri wemunhu.Muyedzo unonzwisisika uye wakavimbika unogona kuona kunyangwe huwandu hudiki hweendotoxin, zvichiita kuti ive chishandiso chakakosha chekuona kuchengetedzeka kwezvigadzirwa izvi.
TAL/LAL reagent ndiyo lyophilized amebocyte lysate inotorwa muropa rebhuruu reLimulus polyphemus kana Tachypleus tridentatus.
Endotoxins ndeye amphiphilic lipopolysaccharides (LPS) iri kunze kwesero membrane ye gram-negative bacteria.Zvigadzirwa zvevabereki zvakasvibiswa nemapyrogens anosanganisira LPS zvinogona kutungamira mukukura kwefivha, kuiswa kwemhinduro yekuzvimba, kuvhunduka, kutadza kwenhengo uye kufa mumunhu.
Naizvozvo, nyika dzepasirese dzakagadzira mirau, inoda kuti chero chigadzirwa chemushonga chinoti chine mbereko uye chisiri-pyrogenic chinofanira kuongororwa chisati chaburitswa.Gel-clot TAL assay yakatanga kugadzirwa yebhakitiriya endotoxins bvunzo (kureva BET).
Nekudaro, dzimwe nzira dzemberi dzeTAL assay dzakabuda.Uye idzi nzira hadzingozongoona chete asiwo kuenzanisa kuvepo kwe endotoxins mumuenzaniso.Kunze kweiyo gel-clot tekinoroji, matekiniki eBET zvakare ane turbidimetric tekinoroji uye chromogenic maitiro.Bioendo, yakatsaurirwa kuona endotoxin, ndiye mugadziri ane hunyanzvi kunyatso gadzira chromogenic TAL/LAL assay.
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay) inopa kukurumidza kuyerwa kwe endotoxin quantification.
Isu tinopawo Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) uye incubation microplate muverengi ELx808IU-SN, iyo inogona kuve nechokwadi chekuvimbika uye kushanda zvakanaka kwekuedza kwako.
Ndezvipi zvinhu zvekinetic chromogenic endotoxin test assaykuyedza endotoxins mumasampuli?
Iyo kinetic chromogenic endotoxin test assay ndiyo imwe nzira inoshandiswa kuyedza endotoxins mumasampuli.Iine zvinhu zvakawanda:
1. Kinetic kuyerwa: Kufanana neye turbidimetric assay, kinetic chromogenic assay inosanganisirawo kinetic kuyerwa.Inovimba nekuita pakati peendotoxins uye chromogenic substrate kugadzira chigadzirwa chine ruvara.Shanduko yekusimba kweruvara nekufamba kwenguva inotariswa, zvichibvumira kuwanda kweiyo endotoxin yakatarisana mumuenzaniso.
2. High senitivity: The kinetic chromogenic assay inonyanya kuoma uye inogona kuona yakaderera endotoxins mumasampuli.Iyo inogona kunyatso kuyera endotoxin kuwanda, kunyangwe pamazinga akaderera kwazvo, kuve nechokwadi chekuonekwa kwakavimbika uye kuwanda.
3. Wide dynamic range: The assay ine hupamhi hunochinja, huchibvumira kuyerwa kwe endotoxin concentrations pane yakakura spectrum.Izvi zvinoreva kuti inogona kuyedza masampula ane akasiyana mazinga endotoxins, anogara ari maviri akaderera uye akakwira kutariswa pasina kudiwa kwesample dilution kana kuisa pfungwa.
4. Mhedzisiro yekukurumidza: The kinetic chromogenic assay inopa mhinduro dzinokurumidza kana ichienzaniswa nemaitiro echinyakare.Iyo inowanzo ine ipfupi yekuyedza nguva, ichigonesa kukurumidza kuyedzwa uye kuongororwa kwemasampuli.Iko kukura kwemavara kunogona kutariswa munguva chaiyo-nguva, uye mhedzisiro inogona kuwanikwa mukati memaminitsi mashoma kusvika maawa akati wandei, zvichienderana neassay kit uye michina inoshandiswa.
5. Otomatiki uye kumisikidza: Iyo assay inogona kuitwa uchishandisa otomatiki masisitimu, senge microplate kuverenga kana.
endotoxin-specific analyzers.Izvi zvinobvumira kuongororwa kwepamusoro-soro uye kunovimbisa kuenderana uye kuyerwa kwakaenzana, kuderedza kukanganisa kwevanhu uye kuwedzera kushanda.
6. Kuenderana nemhando dzakasiyana-siyana dzemuenzaniso: Kinetic chromogenic assay inopindirana nemhando dzakasiyana-siyana dzemhando dzemuenzaniso, kusanganisira mishonga, mishonga yezvokurapa, biologics, uye sampuli dzemvura.Iyo inzira inochinja-chinja inogona kushandiswa kune akasiyana maindasitiri uye mashandisirwo uko endotoxin bvunzo inodiwa.
Pakazere, iyo kinetic chromogenic endotoxin test assay inopa inonzwisisika, inokurumidza, uye yakavimbika nzira yekuona uye kuverengera.
endotoxins mumasampuli.Inoshandiswa zvakanyanya mumishonga, biotechnology, uye hutano maindasitiri ekutonga kwemhando uye kuchengetedza.
ongororo zvinangwa.
Nguva yekutumira: Jul-29-2019
