Iyo bhakitiriya endotoxin bvunzo (BET) inoitwa mumarabhoritari mazhinji emazuva ano pasi pemamiriro akadzora sechinhu chakakosha kudzivirira kukanganiswa.
Zvakakodzeraaseptic nzirayakakosha pakugadzirira nekudzikisa zviyero uye masampula ekubata.Gowningdzidzira kunze kwezvakajairwa marabhoritari yekuzvidzivirira nazvo (PPE) zvinodiwa hazvinetse kunze kwekunge chigadzirwa chiri pasi pekuyedzwa chichida kutariswa kwekuchengetedza muongorori nekuda kwehupfu kana kutapukira.Magirovhosiinofanira kunge isina TALC-isina, sezvo TALC inogona kunge iine akakosha mazinga endotoxins.Vaverengi vendiro, mabhati emvura, uye zvidhinha zvekupisa zvakaomainoshandiswa kuenzanisa incubation inofanira kunge iri pabhenji rerabhoritari kure nekudziya, kufefetedza, uye air conditioning (HVAC) ducts, vibration yakakosha, uye traffic yerabhoritari inogona kukanganisa mhinduro dzebvunzo.Sample kubata nguva uye mamiriroinofanirwa kuve yakatemerwa uye yozonyorwa, kana zvichidikanwa, kuve nechokwadi chekuti mhedzisiro yebvunzo inogona kugadzirwa munguva inokodzera.
Semuyenzaniso, kana marabhoritari ikagamuchira Mvura Yejekiseni (WFI) kana kuti ichiri kushandiswa, inofanira kuiswa mufiriji here kana kuti inogona kuramba iri patembiricha yepakamuri, uye kwenguva yakareba sei?Usati waedzwa, zvinokurudzirwa kuti yekutanga sampu chigaba (s) chisanganiswe zvakaringana usati wabvisa bvunzo aliquot (s) kuti ingave yakananga kuyedzwa kana kunotevera dilution.
Bioendo Bacterial Endotoxin Test, zviedzo izvi zvinosanganisiragel clot nziraendotoxin test assay uyequantitative endotoxin test assay, gel clot nzira endotoxin test assay is qualitative endotoxin kuonekwa, kuedza uku kunoda zvinoshandiswa ndeye depyrogenation processing, zvakadai se endotoxin free reaction tubes, dilution tubes uye pyrogen free tips;quantitative endotoxin yekuona ine kinetic chromogenic endotoxin bvunzo, kinetic turbidimetric endotoxin bvunzo, kuyedza uku kunoda zvinodyiwa zvinofanirwa kusangana nepamusoro peiyo endotoxins isingasviki.0.005EU/ml( 0.001EU/ml), zvakadai se endotoxin isina machubhu, pyrogen isina mazano, uye pyrogen isina microplates, kunyange pyrogen isina resevoir.Nenzira, kana sampuli kurapwa, mudziyo unofanirwa kunge uri endotoxin yemahara bhodhoro remuenzaniso.
Mukuyedzwa kwe endotoxin, kupindira kunogona kusimuka kubva kwakasiyana masosi, senge sampuli matrix zvikamu, bvunzo reagents, kana michina.
Kuti udzivise kukanganiswa kwekuedza, matanho anotevera anogona kutorwa:
1. Kugadzirira Kwemuenzaniso: Kugadzirira kwakakodzera kwemuenzaniso kwakakosha pakuongorora kwakarurama endotoxin.
Iyo sampuli matrix inofanirwa kunyatsoongororwa uye kugadziridzwa kuti ive nechokwadi chekuenderana neye endotoxin assay.
Kunyanya, zvinokanganisa zvinhu zvakaita se lipids uye mapuroteni zvinofanirwa kubviswa kana kuderedzwa uchishandisa nzira dzakakodzera dzakadai sefiltration kana centrifugation.
2. Zvakanaka uye Negative Kudzora: Zvakakosha kuisa maitiro akanaka uye asina kunaka mukuedza kutarisa kupindira.
Positive controls inosimbisa mashandiro ebvunzo, ukuwo zvidzoreso zvisina kunaka zvinoona chero kusvibiswa kana kukanganiswa kubva kune zvikamu zveyedzo.
3. Kudzora Kwehutano: Kudzora kwehutano kunofanira kuitwa pane zvose reagents, midziyo, uye mvura inoshandiswa mukuedza.
Izvi zvinoita kuti mareagents asununguke kubva mukusvibiswa kwe endotoxin uye ari kushanda nemazvo.
4. Kumira: Chiyero chinofanirwa kuve chakamisikidzwa kuti ive nechokwadi chekuti mhedzisiro yese inofananidzwa uye inogona kudhindwa.
Izvi zvinosanganisira kushandiswa kwechivharo chakajairwa kuenzanisa kuyerwa uye kushandiswa kwemaitiro akamisikidzwa ekugadzirira sampuli, incubation, uye kuona.
5. Kusimbiswa: The assay inofanira kusimbiswa kuti ive nechokwadi chokuti ndeyechokwadi, inonzwisisika, uye yakavimbika.
Izvi zvinosanganisira kuyedza huwandu hwemasamples, kusanganisira ayo anozivikanwa kuve ane endotoxin, kuona huchokwadi uye nemazvo ebvunzo.
Nekutevera nhanho idzi, kupindira kunogona kudzikiswa, uye kwakaringana endotoxin kuyedzwa kunogona kuwanikwa.
Nguva yekutumira: Zvita-01-2022
