Mukushanda kwebhakitiriya endotoxin test assay, kushandiswa kwemvura isina endotoxin kwakakosha kudzivirira kusvibiswa.Kuvapo kwe endotoxins mumvura kunogona kutungamirira kumigumisiro isina kururama uye kukanganisa maitiro ekuedza.Apa ndipo panopinda mvura yeLyophilized Amebocyte Lysate (LAL) uye bhakitiriya endotoxin bvunzo (BET) mvura.Idzi mvura dzakanyatsogadzirwa dzakakosha mukuona kuvimbika uye chokwadi chekuyedzwa kwe endotoxin mumaindasitiri akasiyana, anosanganisira mishonga, michina yekurapa, marabhoritari ekutsvagisa uye zvichingodaro.
TheLAL reagent mvuraimvura yakacheneswa zvakanyanya yakanyatsogadzirirwa kushandiswa muyedzo yeLAL ye endotoxins.Iyi mvura inopinda muhurongwa hwekugadzira hwakasimba kuti ive nechokwadi chekuti haina kubva kune endotoxins, iyo inogona kukanganisa mhinduro dzebvunzo.Kusavapo kwema endotoxins mumvura yeLAL reagent kwakakosha mukuvimbisa kunzwisiswa uye kujeka kweiyo LAL bvunzo, zvichiita kuti ive sarudzo yakanaka yekuona endotoxin.
Saizvozvo, BET mvura zvakare chinhu chakakosha muiyo bhakitiriya endotoxin test assay.Mvura iyi yakanyatsogadzirirwa uye kuedzwa kuti ive nechokwadi chekuti haina endotoxins uye zvimwe zvinosvibisa zvinogona kukanganisa chokwadi chebvunzo.Kushandisa mvura yeBET muendotoxin test assay kwakakosha kuti uwane mhedzisiro yakavimbika uye inodzokororwa, sezvo inobvisa njodzi yenhema dzenhema kana nhema dzisina kunaka dzinogona kuitika nekuda kwekuvapo kwe endotoxins mumvura yenguva dzose.
Kukosha kwekushandisa endotoxin-isina mvura mune endotoxin test assay haigone kuwedzeredzwa.Kurongeka uye kuvimbika kwemhedzisiro yebvunzo zvinoenderana nemhando yemvura inoshandiswa.Kuvapo kwe endotoxins mumvura kunogona kutungamira kukuverenga kwenhema, izvo zvinogona kuve nemhedzisiro yakakomba mumaindasitiri uko endotoxin kuongororwa kwakakosha kuti uve nechokwadi chekuchengetedza uye kushanda kwezvigadzirwa.Naizvozvo, kuisa mari muLAL reagent mvura kana BET mvura kwakakosha kuchengetedza kutendeseka kweiyo endotoxin yekuyedza maitiro uye kuve nechokwadi chemhando uye kuchengetedzeka kwezvigadzirwa.
Mukupedzisa, kushandiswa kwemvura isina endotoxin, yakadai seLAL reagent mvura uye BET mvura, yakakosha mukushanda kwebhakitiriya endotoxin test assay.Idzi mvura dzakagadzirwa zvakagadzirirwa kubvisa njodzi yekusvibiswa uye kuve nechokwadi chechokwadi uye kuvimbika kwekuyedzwa kwe endotoxin.Nekushandisa mvura idzi, maindasitiri anogona kuita bvunzo dze endotoxin nechivimbo pasina kutya kwemhedzisiro nekuda kwekuvapo kwe endotoxins mumvura.Pakupedzisira, kushandiswa kweLAL reagent mvura uye BET mvura kwakakosha kusimudzira zviyero zvepamusoro zvemhando uye chengetedzo mumaindasitiri uko endotoxin kuyedzwa kwakakosha zvakanyanya.
Paunenge uchiitisa hutachiona endotoxin test assay, zvakakosha kushandisa endotoxin-isina mvura kuve nechokwadi chechokwadi uye chakavimbika mhedzisiro.
Endotoxins zvinhu zvakadzikama zvekupisa kwesero madziro e gram-negative mabhakitiriya, uye anogona kukonzera fivha, kuvhunduka, uye kunyange kufa muvanhu nemhuka.
Naizvozvo, zvakakosha kushandisa mvura isina endotoxins paunenge uchiita bvunzo.
Kune akati wandei marudzi emvura anogona kushandiswa mune bhakitiriya endotoxin bvunzo assay, kusanganisira LAL reagent mvura, TAL reagent mvura, uye mvura ine depyrogenation kurapwa.Imwe neimwe yemhando idzi dzemvura yakagadzirirwa kuve nechokwadi kuti endotoxins haipo, nekudaro kuve nechokwadi chechokwadi chemhedzisiro yeassay.
LAL reagent mvura imvura yakaedzwa chaizvo uye yakasimbiswa kuti haina endotoxins.Mvura iyi inowanzoshandiswa muLyophilized Amebocyte Lysate (LAL) assay, iyo ndiyo nzira inonyanya kuzivikanwa yekuona endotoxins.Nekushandisa LAL reagent mvura muyedzo, vaongorori vanogona kuva nechivimbo chekuti mvura yacho pachayo haisi kupa kune chero nhema dzakanaka kana nhema dzisina kunaka mhedzisiro.
Saizvozvo, TAL reagent mvura imvura yakaedzwa uye yakasimbiswa kuti haina kubva kune endotoxins.Mvura iyi inowanzoshandiswa muTachypleus Amebocyte Lysate (TAL) assay, imwe nzira yakajairika yekuona endotoxins.Nekushandisa TAL reagent mvura muyedzo, vaongorori vanogona kuva nechivimbo chekuti mvura yacho pachayo haisi kupa kune chero nhema dzakanaka kana nhema dzisina kunaka mhedzisiro.
Mvura ine depyrogenation kurapwa ndiyo imwe sarudzo yekuona kuti mvura inoshandiswa mubhakitiriya endotoxin test assay haina kubva kune endotoxins.Depyrogenation kurapwa kunosanganisira kubviswa kana kusashanda kwemapyrogens, kusanganisira endotoxins, kubva mumvura.Izvi zvinogona kuwanikwa kuburikidza nemaitiro akadai sefiltration, distillation, kana makemikari kurapwa.Nokushandisa mvura ine depyrogenation kurapwa mune assay, vatsvakurudzi vanogona kuva nechivimbo chokuti mvura pachayo haisi kupa kune chero nhema dzakanaka kana nhema dzisina kunaka.
Saka, nei zvakakosha kushandisa endotoxin-isina mvura mune bhakitiriya endotoxin test assay?Kuvapo kwe endotoxins mumvura inoshandiswa mukuedza kunogona kutungamirira kumigumisiro isina kururama, iyo inogona kuva nemigumisiro yakakomba kune zvose tsvakurudzo uye kliniki yekushandiswa.Semuenzaniso, kana endotoxins iripo mumvura, inogona kutungamirira kumigumisiro yakanaka yenhema, zvichiratidza kuvapo kwe endotoxins pavanenge vasipo.Izvi zvinogona kukonzera kunetseka kusiri madikanwa uye kushandisa zvisina kunaka zviwanikwa kugadzirisa nyaya isipo.
Sezvineiwo, kana endotoxins iripo mumvura uye isingaonekwe, inogona kutungamirira kumigumisiro yakaipa yenhema, zvichiratidza kuti endotoxins haipo pavanenge vari.Izvi zvinogona kutungamira mukuburitswa kwezvigadzirwa zvakasvibiswa, zvichiisa hutano hwevanhu nemhuka panjodzi.
Pamusoro peiyo inogona kukanganisa kurongeka kwemhedzisiro yebvunzo, kushandisa mvura isina endotoxin-isina kunogona kukanganisa kuita kwebvunzo pachayo.Endotoxins inogona kukanganisa reagents uye zvishandiso zvinoshandiswa muyedzo, zvichitungamira kune kusavimbika kana kusawirirana mhedzisiro.Nekushandisa endotoxin-isina mvura, vaongorori vanogona kudzikisira njodzi idzi uye kuona kuti assay inoitwa pasi pemamiriro akavimbika.
Pakupedzisira, kuve nechokwadi chekuti mvura inoshandiswa mubhakitiriya endotoxin test assay haina kubva kune endotoxins kwakakosha kuchengetedza kutendeseka kwemhedzisiro yeassay.Ingave uchishandisa LAL reagent mvura, TAL reagent mvura, kana mvura ine depyrogenation kurapwa, vaongorori vanogona kutora matanho ekuita kuti vaone kuti mvura haibatsire kune chero zvisizvo kana kusawirirana mumhedzisiro yeassay.Nekuita izvi, vanogona kuve nechivimbo muchokwadi chezvavakawana uye vanogona kuita sarudzo dzine ruzivo zvichienderana nemhedzisiro yemuyedzo.
Nguva yekutumira: Jan-26-2024
