Endotoxin-isina mvura inoita basa rakakosha mukurongeka uye kuvimbika kweiyo endotoxin test assay operation.Endotoxins, inozivikanwawo se lipopolysaccharides (LPS), zvinhu zvine muchetura zviripo mumadziro emadziro eGram-negative bacteria.Izvi zvinosvibisa zvinogona kukonzera kukuvadza kwakakomba kuvanhu nemhuka kana zvikasabviswa muzvigadzirwa zvekurapa zvakaita semajekiseni, mishonga, uye michina yekurapa.
Kuti uone uye uverenge mazinga endotoxin nenzira kwayo, iyo endotoxin bvunzo inotsamira pane inonzwisisika bvunzo inoda kushandiswa kwemvura isina endotoxin.Iyi mhando yemvura inorapwa kubvisa zvese zvinongedzo zve endotoxins, kuve nechokwadi chekuti chero mhedzisiro yakanaka inogadzirwa neassay inokonzerwa chete nekuvapo kwe endotoxins mumuenzaniso uri kuongororwa, uye kwete mhedzisiro yekusvibiswa kubva mumvura.
Kushandisa endotoxin-isina mvura kunobatsirawo kuderedza mhedzisiro yenhema, izvo zvinogona kuitika kana paine kuteedzera huwandu hweendotoxins mumvura inoshandiswa muyedzo.Izvi zvinogona kutungamira kune mhedzisiro isiriyo, zvichikonzera kunonoka mukuburitswa kwechigadzirwa uye nyaya dzekutonga.
Muchidimbu, endotoxin-isina mvura chinhu chakakosha cheendotoxin test assay mashandiro, kuve nechokwadi chechokwadi uye kuvimbika kweiyi bvunzo yakakosha.Nekudzikisa njodzi yekunyepa uye kuve nechokwadi chekuti mhedzisiro yakanaka inongogadzirwa pamberi peiyo endotoxin kusvibiswa, mvura isina endotoxin inoita basa rakakosha mukuona kuti zvigadzirwa zvekurapa zvakachengeteka uye zvinoshanda kuti zvishandiswe muvarwere.
Bhakitiriya endotoxin bvunzo mvura
Musiyano uripo pakati pebhakitiriya endotoxin yekuyedza mvura uye isina kubereka mvura yejekiseni: pH, bhakitiriya endotoxin uye kupindira zvinhu.
Bhakitiriya endotoxin bvunzo mvura
Musiyano uripo pakati pebhakitiriya endotoxin yekuyedza mvura uye isina kubereka mvura yejekiseni: pH, bhakitiriya endotoxin uye kupindira zvinhu.
1. pH
Iyo yakanyanya kufanirwa pH yemaitiro pakatiLAL reagentuye endotoxin ndeye 6.5-8.0.Naizvozvo, muyedzo yeLAL, United States, Japan Pharmacopoeia uye 2015 edition yeChinese Pharmacopoeia inotara kuti pH kukosha kwechigadzirwa chebvunzo kunofanirwa kugadziridzwa kusvika 6.0-8.0.Iyo pH kukosha kwemvura yekuongororwa kwebhakitiriya endotoxin inowanzodzorwa pa5.0-7.0;iyo pH kukosha kwemvura isina utachiona yejekiseni inofanira kudzorwa pa5.0-7.0.Sezvo mishonga mizhinji isina kusimba acidic, iyo pH kukosha kwemvura yekuongororwa kwebhakitiriya endotoxin yakanaka kune endotoxin test assay kana Lyophilized amebocyte lysate test assay.
2. Bhakitiriya Endotoxin
Kuwanda kwe endotoxin mumvura yekuongorora kwebhakitiriya endotoxin inofanira kunge iri pasi pe 0.015EU pa 1ml, uye huwandu hwe endotoxin mumvura yekuongorora kwebhakitiriya endotoxin muhuwandu hunofanira kuva pasi pe0.005EU pa1ml;Mvura isina utachiona yejekiseni inofanira kunge iine isingasviki 0.25 EU ye endotoxin pa 1ml.
Iyo endotoxin mumvura yebhakitiriya endotoxin bvunzo inofanirwa kunge yakadzikira zvekuti haifanire kukanganisa mhinduro dzebvunzo.Kana mvura isina utachiona yejekiseni ikashandiswa pachinzvimbo chemvura yekuyedza yeEndotoxin bvunzo, nekuda kweiyo yakakwira endotoxin iri mumvura isina majekiseni, mvura isina majekiseni ejekiseni uye Iyo yepamusoro ye endotoxin mumuenzaniso wakaedzwa inogona kuburitsa manyepo, zvichikonzera kurasikirwa kwakananga kwehupfumi. kune bhizinesi.Nekuda kwemusiyano mune endotoxin yemukati, hazvigoneke kushandisa mvura isina hutachiona hwejekiseni pachinzvimbo chekuongorora mvura ye endotoxin test assay kana Lyophilized amebocyte lysate test assay.
3. Kupindira Zvinhu
Mvura yekuongorora kwebhakitiriya endotoxin haifanire kukanganisa iyo LAL reagent, control standard endotoxin uye LAL bvunzo;hapana chinodiwa chemvura isina chibereko chejekiseni.Mvura isina mbereko yejekiseni inoda kuchengetedzeka uye kugadzikana, asi mvura isina utachiona yejekiseni inokanganisa chiitiko uye kugadzikana kwebhakitiriya kutonga standard endotoxin?Mvura Yakasimwa Yejekiseni Inovandudza kana Kurambidza Endotoxin Muedzo?Vashoma vanhu vakaita tsvakiridzo yenguva refu pane izvi.Zvakaongororwa kuburikidza nekuferefeta kuti imwe mvura isina mbereko yejekiseni ine simba rekudzivirira pabvunzo yeLAL.Kana mvura isina utachiona yejekiseni ikashandiswa pachinzvimbo chemvura yekuyedza yeLAL bvunzo, zvakaipa zvenhema zvinogona kuitika, zvichikonzera kupotsa kuonekwa kwe endotoxin, iyo inotyisidzira zvakananga kuchengetedzwa kwemushonga.Nekuda kwekuvapo kwekuvhiringidza zvinhu zvemvura isina majekiseni, hazvigoneke kushandisa mvura isina majekiseni pachinzvimbo chekuongorora mvura yekuongororwa kweLAL.
Kana iko kurongeka kwemvura yekugeza, nzira yekugeza uye mvura yekuyedza inogona kuve nechokwadi, mukana wekuti kutonga kwakanaka muLimulus bvunzo hakugone kusimbiswa chaiko haupo, kunze kwekunge chiyero chinoshandiswa chisina kumira.Kuti tive nechokwadi chechokwadi chemhedzisiro yebvunzo, isu tinofanirwa:
a.Kujairana nezviyero uye maindasitiri maitiro;
b.Shandisa zvigadzirwa zvinokwanisa uye zvigadzirwa zvakajairika;
c.Shanda zvakanyatsoenderana nemaitiro ekushanda.
Nguva yekutumira: Jul-26-2023
