Pyrogen yemahara pipette matipi - Endotoxin yemahara bvunzo machubhu / matipi / microplates

Pyrogen-isina consumables inodyiwa isina exogenous endotoxin, inosanganisira pyrogen-isina pipette matipi (tip bhokisi), pyrogen-isina machubhu ebvunzo kana anonzi endotoxin isina magirazi machubhu, pyrogen-isina girazi ampoules, endotoxin-isina 96-tsime microplates, uye endotoxin- mvura yemahara (kushandiswa kwemvura yedepyrogenated mune yebhakitiriya endotoxin bvunzo), endotoxin isina buffer uye etc. Pakati pavo, mvura yebhakitiriya endotoxintest negel clot nzira uye uwandu endotoxin test assay mune zvose zvine chokuita nePharmacopoeia editions (USP, EP, BP, JP uye China Pharmaceutica). Inoreva mvura isina utachiona yejekiseni ine endotoxin yemukati isingasviki 0.015EU/ml. Ikozvino iyo yazvino vhezheni yePharmacopoeia, iyo BET mvura isingasviki 0.005EU/ml. Kunyangwe iyo yepamusoro mwero isingasviki 0.001EU/ml inokwanisa kugadzirwa uye kupihwa neBioendo.

Rondedzero yacho inopa yakadzama yezvekushandisa-isina pyrogen uye mashandisiro azvo, kunyanya kusimbisa basa ravo rakakosha mukuyedzwa kwe endotoxin pane akasiyana pharmacopoeia zviyero. Heino pfupiso yemapfungwa akakosha:

1. Tsanangudzo:
   • Pyrogen-isina consumables zvinhu zvisina exogenous endotoxins, kusanganisira:
     • Pyrogen-isina pipette mazano(tip box).
     • Endotoxin-isina bvunzo machubhu kana magirazi machubhu.
     • Endotoxin-isina girazi ampoules.
     • Endotoxin-isina 96-tsime microplates.
     • Endotoxin-isina mvura (depyrogenated water).
     • Endotoxin-isina buffers.
2. Mvura yeBacterial Endotoxin Test (BET):
   • Inoshandiswa munzira dzegel clot uye kuwanda endotoxin bvunzo seUSP, EP, BP, JP, uye China Pharmacopeia zviyero.
   • Muyero wepakutanga: ≤0.015 EU/ml yemvura isina utachiona yejekiseni.
   • Chinodiwa ikozvino (yazvino pharmacopoeia): ≤0.005 EU/ml yeBET mvura.
   • Yepamberi mwero: Bioendo inogona kugadzira mvura ine ≤0.001 EU/ml endotoxin yemukati, ichisangana nemhando yepamusoro zvinodiwa.

Mazano ekunatsa:

• Simbisa huwandu hwekushandisa, semuenzaniso, marabhoritari, mishonga yemishonga, kana biotech.
• Ipa humwe humbowo hwehunyanzvi hweBioendo mukugadzira kusimbisa kukwikwidza kwayo.
• Simbisa mabhenefiti chaiwo ezvigadzirwa zveBioendo zvemhando yepamusoro, senge kukwidziridzwa kweyeyedzo kana kutevedzera mutemo.

Ungade here kuti ndibatsire kugadzira izvi kuita chimwe chigadzirwa kana tsananguro yehunyanzvi?

Ane hukama akakosha mazwi e endotoxins, uye endotoxin yemahara consumables, pyrogen uye kupisa masosi ari maviri akasiyana pfungwa:Pyrogen: Uyewo inonzi pyrogen kana exothermic factor. zvinhu zvinogona kukonzera kuwedzera kwekushisa kwemuviri.Heat Source: Chinhu chinoburitsa kupisa. Zvakadai sokupisa machisi, marasha, etc.Iyo inonzi "non-pyrogenic consumables" uye "pyrogenic response" yevamwe vanogadzira uye vatengesi chaizvoizvo mazita asina ruzivo uye anotsausa. Izvo zvakakodzera zvinofanirwa kunge zviri "Pyrogen Yemahara" uye "Pyrogen Response".

Nei Pyrogen yemahara inodyiwa ichidikanwa muye endotoxin bvunzo assay, ese ari maviri gel clot endotoxin test assay uye kuwanda endotoxin test assay?

Hongu, pyrogen-free consumables inokosha pakuita endotoxin test assay zvakarurama uye zvakavimbika.Kuvapo kwepyrogens, izvo zvinhu zvinopisa fivha zvinowanzobva kune bhakitiriya endotoxins, zvinogona kupindira nemigumisiro yekuedza uye kutungamirira kuverenga kwenhema.The Endotoxin bvunzo, inowanzozivikanwa seLimulus amebocyte lysate (LAL) bvunzo kana inonzi Lyophilized amebocyte lysate (LAL) bvunzo, inoshandiswa kuona uye kuyera kuvepo kwebhakitiriya endotoxins mumishonga, zvishandiso zvekurapa, uye zvimwe zvigadzirwa. Muedzo weLAL unovimba nekuita kuri pakati peLAL reagent uye endotoxins kugadzira kugwamba kana chromogenic mhinduro. Kuti uve nechokwadi chemhedzisiro, zvakakosha kushandisa zvinodyiwa zvisina pyrogens. Pyrogens inogona kusvibisa zvinhu zvakasiyana-siyana zverabhoritari, kusanganisiraglassware, pipette matipi, machubhu, uye midziyo yekuenzanisira. Kana zvinodyiwa zvine pyrogen zvakasangana neLAL reagent kana sampuli dzebvunzo, zvinogona kukonzeresa manyepo-zvakanaka maitiro, zvichizotungamira kune mhedziso isiriyo pamusoro pekuvapo kana kuunganidzwa kweendotoxins. Zvishandiso zvisina pyrogen zvinogadzirwa uye zvinoedzwa zvakananga kuderedza kana kubvisa. kuvapo kwe pyrogens. Ivo vanopinda zvakaomesesa zvemhando yekudzora maitiro kuti vave nechokwadi chekuti vanosangana nezvinodiwa zvekuyedzwa kwe endotoxin. Kushandisa izvi zvekushandisa zvine hunyanzvi kunobatsira kuchengetedza kutendeseka uye kurongeka kweye endotoxin test assay, kuve nemhedzisiro yakavimbika uye nekusimudzira kuchengetedzwa kwevarwere mumishonga uye biomedical application.