Machubhu egirazi ane depyrogenation process anodiwa muye endotoxin bvunzo assay kuve nechokwadi chechokwadi uye kuvimbika kwemhedzisiro yebvunzo.Endotoxins inodziya-yakagadzika mamorekuru zvikamu zvekunze kwesero madziro eimwe giremu-negative mabhakitiriya, uye anogona kukonzera kurwara kwakanyanya uye kunyange kufa muvanhu kana aripo mune zvekurapa zvigadzirwa kana zvishandiso.
Kuti ione endotoxins, muyedzo unoshandisa mareagents ane Limulus Amebocyte Lysate (LAL) kana kuti Lyophilized amebocyte lysate, inotorwa kubva mumasero eropa ehakanje ane nzira yekuvhara iyo inogadziriswa ne endotoxins.Nekudaro, machubhu egirazi asina kubviswa depyrogenated anogona kukanganisa iyo LAL test assat nekuita kuti ivhare mashandiro ayo uye kuburitsa mhedzisiro yenhema.Naizvozvo, machubhu egirazi anoshandiswa muye endotoxin bvunzo assay anofanirwa kuve depyrogenated kubvisa chero endotoxins inogona kunge iripo uye kudzivirira activation yeLAL reagent.Izvi zvinova nechokwadi chekuti mhedzisiro yebvunzo ye endotoxin ndeyechokwadi uye yakavimbika uye kuti varwere havasi kuoneswa kune inokuvadza mazinga endotoxins.uye chengetedza kuchengetedza kwemishonga yevabereki mumishonga, mapuroteni, sero tsika, DNA uye zvichingodaro.
Iko kudikanwa kweendotoxin-isina girazi machubhu anoshandisa mune endotoxin yekuona assay mashandiro:
Endotoxin-isina girazi machubhuchinhu chakakosha chechero endotoxin test assay.Aya machubhu egirazi akagadzirirwa kudzikisa njodzi yekusvibiswa kwe endotoxin panguva yekuyedzwa, kuve nechokwadi chemhedzisiro uye yakavimbika mhedzisiro.
Chimwe chezvinhu zvakakosha zveendotoxin-isina girazi machubhu ndiko kuumbwa kwavo kwemakemikari.Aya machubhu anogadzirwa kubva kumhando yepamusoro yegirazi yeborosilicate, iyo inozivikanwa nekusarudzika kwayo kupikisa makemikari ekuora.Izvi zvinovaita kuti vakwanise kushandiswa mukuyedza endotoxin, sezvo vachikwanisa kushingirira kuratidzwa kune akasiyana siyana ebvunzo makomisheni pasina kusvibisa kana kusvibisa sampuli.
Chimwe chakakosha cheendotoxin-isina girazi machubhu kuchena kwavo.Machubhu aya anocheneswa nekucheneswa asati ashandiswa kuti abvise chero angangoita hutachiona.Ivo zvakare vanoyedzerwa zvakasimba kusvibiswa kwe endotoxin, kuve nechokwadi kuti havana kusununguka kubva kune chero huwandu hwechinhu chinokuvadza ichi.
Pamusoro pezvo, endotoxin-isina girazi machubhu akagadzirirwa kuve nyore kushandisa.Iwo anowanzo kuwanikwa muhukuru hwehukuru uye zvigadziriso kuti zvigadzike akasiyana sampuli mavhoriyamu uye nzira dzekuyedza ese ari maviri emhando endotoxin bvunzo assay uye huwandu endotoxin test assay.Izvo zvakare zvinopindirana neakasiyana emhando yekugadzirira uye yekuyedza michina, zvichiita kuti ive inogoneka uye iri nyore sarudzo ye endotoxin yekuyedza marabhoritari.
Pakazere, endotoxin-isina girazi machubhu anoita basa rakakosha mukurongeka uye kuvimbika kwekuyedzwa kweendotoxin.Kuvaka kwavo kwemhando yepamusoro, kuchena, uye nyore kushandisa zvinovaita chinhu chakakosha chechero rakabudirira endotoxin test assay.
Bioendo Endotoxin-isina girazi machubhu ane saizi10*75mm, 12*75mm, 13*100mm uye 16*100mmkune dilutions maitiro uye maitiro ekuita.
Endotoxin-isina machubhu egirazi anosangana neyepamusoro chiyero cheendotoxins isingasviki 0.005EU/ml.
Endotoxin-isina machubhu egirazi anofanirwa kushandiswa mujeli clot endotoxin test assay kudzivirira nhema-zvakanaka mhedzisiro.
Endotoxins ibhakitiriya sero madziro emadziro anogona kusvibisa midziyo yemurabhoritari, kusanganisira machubhu egirazi.
Iyo gel clot endotoxin test assay inoshandiswa kuona kuvepo kwe endotoxins mune sampuli.Muchiyedzo ichi, clot inoumbwa pamberi pe endotoxins.Uku kuumbwa kwevhavha kunozofananidzwa nekutonga kuona iyo endotoxin concentration.
Kushandisa endotoxin-isina girazi machubhu kunobatsira kuve nechokwadi chekuti endotoxin yekuona ndeyechokwadi.Izvi zvinodaro nekuti endotoxins inogona kunamatira kumusoro kwegirazi machubhu uye kukanganisa mhinduro dzeassay.
Kuve nechokwadi chekuti machubhu egirazi anoshandiswa mujeli clot endotoxin test assay haana endotoxin-isina, anofanirwa kugezwa nedetergent uye onyatsosukutwa nemvura isina endotoxin.Pamusoro pezvo, ivo vanofanirwa kuvharirwa vachishandisa autoclaving kana yakaoma kupisa sterilization.
Mukupedzisa, kushandisa endotoxin-isina girazi machubhu mune gel clot endotoxin test assay yakakosha kuve nechokwadi chekuonekwa kwe endotoxins.Machubhu aya anofanira kunyatsocheneswa nekucheneswa kuti abvise chero hutachiona hunogona kuitika.
Nguva yekutumira: Jun-02-2023
