Chromogenic tekinoroji iri pakati pematanho matatu anewo gel-clot technique uye turbidimetric nzira yekuona kana kuyera endotoxins kubva kuGram-negative mabhakitiriya nekushandisa amoebocyte lysate yakatorwa kubva muropa rebhuruu rehorshoe crab (Limulus polyphemus kana Tachypleus tridentatus).Inogona kuverengerwa seyekupedzisira-chromogenic assay kana kinetic-chromogenic assay yakavakirwa pane chaiyo assay musimboti wakashandiswa.
Maitiro ekuita ndeokuti: iyo amebocyte lysate ine cascade ye serine protease enzymes (proenzymes) iyo inogona kushandiswa nebhakitiriya endotoxins.Endotoxins inomutsa maproenzymes kuti agadzire ma enzymes (anonzi coagulase), iyo yekupedzisira inokonzeresa kupatsanurwa kweiyo isina ruvara substrate, ichiburitsa yero-ruvara chigadzirwa pNA.Iyo pNA yakaburitswa inogona kuyerwa photometrically pa405nm.Uye iyo yekunyura inoenderana neiyo endotoxin concentration, ipapo iyo endotoxin yekumisikidza inogona kuverengerwa zvinoenderana.
Nguva yekutumira: Sep-29-2019
